Product Overview
ORG istem is a human induced pluripotent stem cell(hiPSC) line generated from human dermal fibroblasts(HDFa)(Cat No. DFFV001) using an mRNA vector-based reprogramming method.
ORG istem maintains stable expression of key pluripotency markers during expansion culture and demonstrates differentiation potential toward multiple cell lineages, including cardiomyocytes, vascular endothelial cells, neural cells, and skin cells.
Key Features
• Derived from human dermal fibroblasts(HDFa)(Cat No. DFFV001)
• Generated using an mRNA vector-based reprogramming method
• Stable expression of key pluripotency markers
• Demonstrated differentiation potential across multiple cell lineages
• Cell line identity validated by STR analysis
• Viral safety testing completed
Product Highlights
Stable Pluripotency Marker Expression
ORG istem maintains consistently high expression levels of the pluripotency markers SOX2, NANOG, and OCT4 during expansion culture.
Multi-Lineage Differentiation Potential
ORG istem has demonstrated differentiation potential toward cardiomyocytes(TNNT2), vascular endothelial cells(PECAM1), neural cells(TUBB3), and skin cells(KRT5), as confirmed by lineage-specific marker expression.
Designed for Differentiation Research
ORG istem supports the maintenance of pluripotency during expansion and enables the evaluation of lineage-specific gene expression during differentiation. This makes it suitable for studies of cellular differentiation mechanisms and downstream applications.
Culture Protocol Support
Culture and expansion protocols, including weekly passaging procedures using ORG istem culture reagents, are provided to support consistent cell maintenance.
Cell Line Authentication
Cell line identity has been validated by STR analysis.
Viral Testing
Viral testing was completed with negative results for the following viruses:
HIV, HTLV, HPV, HBV, HHV
Applications
1. Basic Stem Cell Research
For studies of hiPSC expansion, maintenance, characterization, and pluripotency.
2. Directed Differentiation Research
For differentiation studies targeting cardiomyocytes, vascular endothelial cells, neural cells, skin cells, and other cell types.
3. Drug Evaluation and Disease Modeling
For drug response and toxicity studies, as well as in vitro disease modeling using differentiated cell types.
Additional Information
The human dermal fibroblasts(Cat No. DFFV001) used to generate ORG istem are also available separately.
Culture protocols using ORG istem culture reagents are provided.
ORG istem is intended for research use. Culture conditions should be optimized according to the intended application and experimental requirements.
Recommended Applications and Users
Pharmaceutical and Biotechnology Research
Preclinical CROs
Stem Cell and Regenerative Medicine Research
Academic and Hospital-Affiliated Research Laboratories
Organoid and Cell Differentiation Research
Product Summary
ORG istem is a human induced pluripotent stem cell(hiPSC) line generated from human dermal fibroblasts(HDFa)(Cat No. DFFV001) using an mRNA vector-based reprogramming method. The cell line maintains stable expression of key pluripotency markers, including SOX2, NANOG, and OCT4, during expansion culture and demonstrates differentiation potential toward cardiomyocytes, vascular endothelial cells, neural cells, and skin cells. ORG istem is suitable for basic stem cell research, directed differentiation studies, regenerative medicine research, disease modeling, and drug evaluation.
